A new real-time PCR test to detect Xylophilus ampelinus
A new real-time PCR test was developed for the specific detection of Xylophilus ampelinus (EPPO A2 List). Real-time PCR enabled a specific and rapid detection of X. ampelinus from plant tissue extracts and was found more sensitive than nested-PCR (at least tenfold more sensitive). It is considered that this new PCR method can be used as a screening test, in addition to isolation on growing media or other methods. The authors concluded that real-time PCR could provide a rapid and specific identification of isolated colonies, as well as a relative quantification of X. ampelinus bacteria.
Dreo T, Gruden K, Manceau C, Janse, JD, Ravnikar M (2007) Development of a real-time PCR-based method for detection of Xylophilus ampelinus. Plant Pathology 56(1), 9-16.