Surveys on Globodera species in Lithuania
In 2006, studies were carried out in Lithuania to analyze Globodera species occurring in potato fields using morphological and molecular methods. So far, only Globodera rostochiensis is known to occur in Lithuania (EPPO A2 List; see EPPO RS 2005/075 and 2004/075). 11 406 soil samples were collected from a total area of 2 742 ha (across the 10 counties of Lithuania). A total of 672 cysts were recovered and on the basis of morphological characters, only G. rostochiensis was identified. In addition, 117 cysts from 5 counties (Vilnius, Kaunas, Šiauliai, Telšiai and Utena) were identified by PCR using specific primer pairs which enable G. rostochiensis and G. pallida to be distinguished. Again, only G. rostochiensis was identified. The nematode was found in 116 fields out of 437, mainly in small fields cultivated with various plant species and located near homesteads. It is concluded that both morphological and molecular methods confirm the presence of G. rostochiensis and the absence of G. pallida in Lithuania.
Jogaitė V, Čėpulytė R, Stanelis A, Būda V (2007) Monitoring of Globodera spp. in Lithuania using diagnostic morphometric analysis and Polymerase Chain Reaction. Acta Zoologica Lituanica 17(2), 184-186.