Macroarray detection of potato viruses and Potato spindle tuber viroid
In the USA, a macroarray was developed for the simultaneous detection of 11 potato viruses and 1 viroid: Alfalfa mosaic virus (Alfamovirus), Cucumber mosaic virus (Cucumovirus), Potato mop-top virus (Pomovirus), Potato leafroll virus (Polerovirus), Potato latent virus (Carlavirus), Potato virus A (Potyvirus), Potato virus M (Carlavirus), Potato virus S (Carlavirus), Potato virus X (Potexvirus), Potato virus Y (Potyvirus), Tobacco rattle virus (Nepovirus), Potato spindle tuber viroid (Pospiviroid, EPPO A2 List). The objectives were to develop and validate a new diagnostic tool for the pathogens which are normally included in North American potato seed certification programs and to make progress in the development of new methodologies for simultaneously testing pathogens of solanaceous crops. It was concluded that this macroarray usefully complemented the existing serological tests but could not be proposed as a replacement for them yet. However, it was considered that, in the longer term, the microarray will offer a cost-effective approach for the detection of multiple pathogens in solanaceous crops.
Sources
Agindotan B, Perry KL (2008 Macroarray detection of eleven potato-infecting viruses and Potato spindle tuber viroid. Plant Disease 92(5), 730-740.