Direct detection of Erwinia amylovora in plant tissues by PCR
A new PCR test for the detection of Erwinia amylovora (EPPO A2 List) has been developed in Austria. It uses a modified commercial PCR kit which allows a direct detection of the bacterium in plant tissues. This new PCR method was applied to 951 plant samples collected in Austria and compared with the standard protocol currently used (based on EPPO Standard PM 7/20, 2004). The new PCR method was found highly sensitive, rapid, easy to use, and therefore appropriate for the routine testing of large numbers of samples.
Sources
Stöger A, Schaffer J, Ruppitsch W (2006) A rapid and sensitive method for direct detection of Erwinia amylovora in symptomatic and asymptomatic plant tissues by Polymerase Chain Reaction. Journal of Phytopathology 154(7-8), 469-473.