Use of high performance capillary electrophoresis to differentiate pathotypes of Globodera rostochiensis and G. pallida
Pathotypes of Globodera rostochiensis and G. pallida (EPPO A2 quarantine pests) are traditionally differentiated by their reproductive ability on a standard set of potato cultivars which contain different resistance genes to these nematodes. A technique of high performance capillary electrophoresis has been tested on a collection of different nematodes populations, and it provided polypeptide profiles which allow differentiation between pathotypes of Globodera rostochiensis and G. pallida (Ro1, Ro2, Ro3, Ro4, Ro5 and Pa1, Pa2/3). The authors felt that this simple reliable method will provide a useful tool to differentiate pathotypes of potato cyst nematodes with minimum sample preparation.
Sources
Hinch, J.M.; Alberdi, F.; Smith, S.C.; Woodward, J.R.; Evans, K. (1998) Discrimination of European and Australian Globodera rostochiensis and G. pallida pathotypes by high performance capillary electrophoresis.
Fundamental and Applied Nematology, 21(2), 123-128.