PCR assay to detect simultaneously Xanthomonas campestris pv. pelargonii and Ralstonia solanacearum in Pelargonium
A multiplex PCR assay has been developed in USA to detect simultaneously the two major bacterial pathogens of geraniums (Pelargonium spp.) which are Xanthomonas campestris pv. pelargonii and Ralstonia solanacearum (EPPO A2 quarantine pest). Using this method, it is also possible to differentiate between the two pathogens. Further work is being done to adapt this technique to larger amounts of samples and to improve its rapidity.
Sources
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Glick, D.L.; Coffey, C.M.; Sulzinski, M.A. (2002) Simultaneous PCR detection of the two major bacterial pathogens of geranium.
Journal of Phytopathology, 150(2), 54-59.
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